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whitematter boundary  (WhiteMatter Labs GmbH)
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visiopharm software v2.12.3.0  (Visiopharm AS)
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Visiopharm Software V2.12.3.0, supplied by Visiopharm AS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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boundary conditions  (ANSYS inc)
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Boundary Conditions, supplied by ANSYS inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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commercial software comsol  (COMSOL Inc)
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momentum transfer of a boltzmann-lattice fluid with boundaries  (Lallemand inc)
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Lallemand inc momentum transfer of a boltzmann-lattice fluid with boundaries
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boundary conditions for analysis of s.i. engine piston using  (ANSYS inc)
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ANSYS inc boundary conditions for analysis of s.i. engine piston using
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doubleclick's plan  (DoubleClick Inc)
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metamorph software  (MetaMorph Inc)
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MetaMorph Inc metamorph software
Determination of MMP-1 lamellipodia localization. (A) Visual demonstration of objective invadopodia boundary determination through depth-coding. (A) Cellular architecture was outlined by Alexa-647-phalloidin staining (pseudo-coloured white) and aided by nuclear counterstaining with DAPI (B-blue). MMP-1 localization (C-red) and the reflected image (D-turquoise). The reflection image interference rings [E-Merge, labelled with asterisk ( * )] delineate the stretched out regions of cellular bodies (lamellipodia) showing striped wave-like formations of protruding cellular frontlines. To demonstrate this phenomena more clearly, the interferential image was pseudo-coloured according to the height of cellular features (depth-coding, F). The <t>boundaries</t> of lamellipodia region circled (G-white). (B) MMP-1 delivery is FTI-276 sensitive. Fifteen to twenty randomly chosen fields of individual images (representing 60–90 individual cells) were captured of each condition with 63χ oil immersion lens on the Zeiss LSM510 confocal microscope. Blue (DAPI, nucleus), red (MMP-1), pseudo-coloured white (F-actin) and reflected interference images (not shown) were collected. MetaMorph analysis was performed on the extracted red channels for total and partial surface areas and pixel counts to determine the podia formation activity and its partial MMP-1 representation as described. Each cell total surface area and MMP-1 content treated as fixed (100%). From this the lamellipodia formation and partial MMP-1 localization were determined as percentage of total values (surface area and red pixel counts). These percentage results presented as bar graph, where untreated cells provide the reference basis ∇ 100% showing for drugs affecting lamellipodia surface area (bar graph on the left) and MMP-1 loading (bar graph on the right). Statistical analysis shows that the quantization of lamellipodia area and MMP-1 loading are different for and by the presented conditions with P ∇ 4.09E−73 and P ∇ 5.60786E−30 confidence levels, respectively.
Metamorph Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ann-phys.org  (Verlag GmbH)
90
Verlag GmbH ann-phys.org
Determination of MMP-1 lamellipodia localization. (A) Visual demonstration of objective invadopodia boundary determination through depth-coding. (A) Cellular architecture was outlined by Alexa-647-phalloidin staining (pseudo-coloured white) and aided by nuclear counterstaining with DAPI (B-blue). MMP-1 localization (C-red) and the reflected image (D-turquoise). The reflection image interference rings [E-Merge, labelled with asterisk ( * )] delineate the stretched out regions of cellular bodies (lamellipodia) showing striped wave-like formations of protruding cellular frontlines. To demonstrate this phenomena more clearly, the interferential image was pseudo-coloured according to the height of cellular features (depth-coding, F). The <t>boundaries</t> of lamellipodia region circled (G-white). (B) MMP-1 delivery is FTI-276 sensitive. Fifteen to twenty randomly chosen fields of individual images (representing 60–90 individual cells) were captured of each condition with 63χ oil immersion lens on the Zeiss LSM510 confocal microscope. Blue (DAPI, nucleus), red (MMP-1), pseudo-coloured white (F-actin) and reflected interference images (not shown) were collected. MetaMorph analysis was performed on the extracted red channels for total and partial surface areas and pixel counts to determine the podia formation activity and its partial MMP-1 representation as described. Each cell total surface area and MMP-1 content treated as fixed (100%). From this the lamellipodia formation and partial MMP-1 localization were determined as percentage of total values (surface area and red pixel counts). These percentage results presented as bar graph, where untreated cells provide the reference basis ∇ 100% showing for drugs affecting lamellipodia surface area (bar graph on the left) and MMP-1 loading (bar graph on the right). Statistical analysis shows that the quantization of lamellipodia area and MMP-1 loading are different for and by the presented conditions with P ∇ 4.09E−73 and P ∇ 5.60786E−30 confidence levels, respectively.
Ann Phys.Org, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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comsol stokes mode  (COMSOL Inc)
90
COMSOL Inc comsol stokes mode
Determination of MMP-1 lamellipodia localization. (A) Visual demonstration of objective invadopodia boundary determination through depth-coding. (A) Cellular architecture was outlined by Alexa-647-phalloidin staining (pseudo-coloured white) and aided by nuclear counterstaining with DAPI (B-blue). MMP-1 localization (C-red) and the reflected image (D-turquoise). The reflection image interference rings [E-Merge, labelled with asterisk ( * )] delineate the stretched out regions of cellular bodies (lamellipodia) showing striped wave-like formations of protruding cellular frontlines. To demonstrate this phenomena more clearly, the interferential image was pseudo-coloured according to the height of cellular features (depth-coding, F). The <t>boundaries</t> of lamellipodia region circled (G-white). (B) MMP-1 delivery is FTI-276 sensitive. Fifteen to twenty randomly chosen fields of individual images (representing 60–90 individual cells) were captured of each condition with 63χ oil immersion lens on the Zeiss LSM510 confocal microscope. Blue (DAPI, nucleus), red (MMP-1), pseudo-coloured white (F-actin) and reflected interference images (not shown) were collected. MetaMorph analysis was performed on the extracted red channels for total and partial surface areas and pixel counts to determine the podia formation activity and its partial MMP-1 representation as described. Each cell total surface area and MMP-1 content treated as fixed (100%). From this the lamellipodia formation and partial MMP-1 localization were determined as percentage of total values (surface area and red pixel counts). These percentage results presented as bar graph, where untreated cells provide the reference basis ∇ 100% showing for drugs affecting lamellipodia surface area (bar graph on the left) and MMP-1 loading (bar graph on the right). Statistical analysis shows that the quantization of lamellipodia area and MMP-1 loading are different for and by the presented conditions with P ∇ 4.09E−73 and P ∇ 5.60786E−30 confidence levels, respectively.
Comsol Stokes Mode, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ansys/ls-dyna software  (ANSYS inc)
90
ANSYS inc ansys/ls-dyna software
Determination of MMP-1 lamellipodia localization. (A) Visual demonstration of objective invadopodia boundary determination through depth-coding. (A) Cellular architecture was outlined by Alexa-647-phalloidin staining (pseudo-coloured white) and aided by nuclear counterstaining with DAPI (B-blue). MMP-1 localization (C-red) and the reflected image (D-turquoise). The reflection image interference rings [E-Merge, labelled with asterisk ( * )] delineate the stretched out regions of cellular bodies (lamellipodia) showing striped wave-like formations of protruding cellular frontlines. To demonstrate this phenomena more clearly, the interferential image was pseudo-coloured according to the height of cellular features (depth-coding, F). The <t>boundaries</t> of lamellipodia region circled (G-white). (B) MMP-1 delivery is FTI-276 sensitive. Fifteen to twenty randomly chosen fields of individual images (representing 60–90 individual cells) were captured of each condition with 63χ oil immersion lens on the Zeiss LSM510 confocal microscope. Blue (DAPI, nucleus), red (MMP-1), pseudo-coloured white (F-actin) and reflected interference images (not shown) were collected. MetaMorph analysis was performed on the extracted red channels for total and partial surface areas and pixel counts to determine the podia formation activity and its partial MMP-1 representation as described. Each cell total surface area and MMP-1 content treated as fixed (100%). From this the lamellipodia formation and partial MMP-1 localization were determined as percentage of total values (surface area and red pixel counts). These percentage results presented as bar graph, where untreated cells provide the reference basis ∇ 100% showing for drugs affecting lamellipodia surface area (bar graph on the left) and MMP-1 loading (bar graph on the right). Statistical analysis shows that the quantization of lamellipodia area and MMP-1 loading are different for and by the presented conditions with P ∇ 4.09E−73 and P ∇ 5.60786E−30 confidence levels, respectively.
Ansys/Ls Dyna Software, supplied by ANSYS inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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periodic ports and boundaries  (COMSOL Inc)
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COMSOL Inc periodic ports and boundaries
Determination of MMP-1 lamellipodia localization. (A) Visual demonstration of objective invadopodia boundary determination through depth-coding. (A) Cellular architecture was outlined by Alexa-647-phalloidin staining (pseudo-coloured white) and aided by nuclear counterstaining with DAPI (B-blue). MMP-1 localization (C-red) and the reflected image (D-turquoise). The reflection image interference rings [E-Merge, labelled with asterisk ( * )] delineate the stretched out regions of cellular bodies (lamellipodia) showing striped wave-like formations of protruding cellular frontlines. To demonstrate this phenomena more clearly, the interferential image was pseudo-coloured according to the height of cellular features (depth-coding, F). The <t>boundaries</t> of lamellipodia region circled (G-white). (B) MMP-1 delivery is FTI-276 sensitive. Fifteen to twenty randomly chosen fields of individual images (representing 60–90 individual cells) were captured of each condition with 63χ oil immersion lens on the Zeiss LSM510 confocal microscope. Blue (DAPI, nucleus), red (MMP-1), pseudo-coloured white (F-actin) and reflected interference images (not shown) were collected. MetaMorph analysis was performed on the extracted red channels for total and partial surface areas and pixel counts to determine the podia formation activity and its partial MMP-1 representation as described. Each cell total surface area and MMP-1 content treated as fixed (100%). From this the lamellipodia formation and partial MMP-1 localization were determined as percentage of total values (surface area and red pixel counts). These percentage results presented as bar graph, where untreated cells provide the reference basis ∇ 100% showing for drugs affecting lamellipodia surface area (bar graph on the left) and MMP-1 loading (bar graph on the right). Statistical analysis shows that the quantization of lamellipodia area and MMP-1 loading are different for and by the presented conditions with P ∇ 4.09E−73 and P ∇ 5.60786E−30 confidence levels, respectively.
Periodic Ports And Boundaries, supplied by COMSOL Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Determination of MMP-1 lamellipodia localization. (A) Visual demonstration of objective invadopodia boundary determination through depth-coding. (A) Cellular architecture was outlined by Alexa-647-phalloidin staining (pseudo-coloured white) and aided by nuclear counterstaining with DAPI (B-blue). MMP-1 localization (C-red) and the reflected image (D-turquoise). The reflection image interference rings [E-Merge, labelled with asterisk ( * )] delineate the stretched out regions of cellular bodies (lamellipodia) showing striped wave-like formations of protruding cellular frontlines. To demonstrate this phenomena more clearly, the interferential image was pseudo-coloured according to the height of cellular features (depth-coding, F). The boundaries of lamellipodia region circled (G-white). (B) MMP-1 delivery is FTI-276 sensitive. Fifteen to twenty randomly chosen fields of individual images (representing 60–90 individual cells) were captured of each condition with 63χ oil immersion lens on the Zeiss LSM510 confocal microscope. Blue (DAPI, nucleus), red (MMP-1), pseudo-coloured white (F-actin) and reflected interference images (not shown) were collected. MetaMorph analysis was performed on the extracted red channels for total and partial surface areas and pixel counts to determine the podia formation activity and its partial MMP-1 representation as described. Each cell total surface area and MMP-1 content treated as fixed (100%). From this the lamellipodia formation and partial MMP-1 localization were determined as percentage of total values (surface area and red pixel counts). These percentage results presented as bar graph, where untreated cells provide the reference basis ∇ 100% showing for drugs affecting lamellipodia surface area (bar graph on the left) and MMP-1 loading (bar graph on the right). Statistical analysis shows that the quantization of lamellipodia area and MMP-1 loading are different for and by the presented conditions with P ∇ 4.09E−73 and P ∇ 5.60786E−30 confidence levels, respectively.

Journal: Journal of Cellular and Molecular Medicine

Article Title: Matrix metalloproteinase-1 contribution to sarcoma cell invasion

doi: 10.1111/j.1582-4934.2011.01402.x

Figure Lengend Snippet: Determination of MMP-1 lamellipodia localization. (A) Visual demonstration of objective invadopodia boundary determination through depth-coding. (A) Cellular architecture was outlined by Alexa-647-phalloidin staining (pseudo-coloured white) and aided by nuclear counterstaining with DAPI (B-blue). MMP-1 localization (C-red) and the reflected image (D-turquoise). The reflection image interference rings [E-Merge, labelled with asterisk ( * )] delineate the stretched out regions of cellular bodies (lamellipodia) showing striped wave-like formations of protruding cellular frontlines. To demonstrate this phenomena more clearly, the interferential image was pseudo-coloured according to the height of cellular features (depth-coding, F). The boundaries of lamellipodia region circled (G-white). (B) MMP-1 delivery is FTI-276 sensitive. Fifteen to twenty randomly chosen fields of individual images (representing 60–90 individual cells) were captured of each condition with 63χ oil immersion lens on the Zeiss LSM510 confocal microscope. Blue (DAPI, nucleus), red (MMP-1), pseudo-coloured white (F-actin) and reflected interference images (not shown) were collected. MetaMorph analysis was performed on the extracted red channels for total and partial surface areas and pixel counts to determine the podia formation activity and its partial MMP-1 representation as described. Each cell total surface area and MMP-1 content treated as fixed (100%). From this the lamellipodia formation and partial MMP-1 localization were determined as percentage of total values (surface area and red pixel counts). These percentage results presented as bar graph, where untreated cells provide the reference basis ∇ 100% showing for drugs affecting lamellipodia surface area (bar graph on the left) and MMP-1 loading (bar graph on the right). Statistical analysis shows that the quantization of lamellipodia area and MMP-1 loading are different for and by the presented conditions with P ∇ 4.09E−73 and P ∇ 5.60786E−30 confidence levels, respectively.

Article Snippet: The boundaries were then traced (G) and areas numbered and quantized with MetaMorph software.

Techniques: Staining, Microscopy, Activity Assay